Volume 4, Issue 3 (3 2006)
sjsph 2006, 4(3): 53-60 |
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Sedaghat M, Dorrani S, Pourshafie M, Saifi M, Ranjbar R. Molecular detection of the pathogenic hlyA gene and antibiotic susceptibility in 100 specimens from patients with cholera. sjsph 2006; 4 (3) :53-60
URL: http://sjsph.tums.ac.ir/article-1-200-en.html
URL: http://sjsph.tums.ac.ir/article-1-200-en.html
Abstract: (8405 Views)
Background and Aim: Cholera is one of the most important endemic and epidemic diseases in our country. Therefore, keeping track of the changes in antibiotic resistance patterns and continuous genotyping and serotyping of the isolates is essential.
Materials and Methods: One hundred stool samples were collected from the patients with cholera in different cities, including Tehran, Kashan, Kermanshah and Ahwaz. The specimens were subjected to a series of diagnostic tests. Strains were identified by serotyping and their antibiotic resistance profiles were defined through susceptibility tests. We also used a polymerase chain reaction (PCR) assay to identify the hly A gene.
Results: Group-specific antisera were used for identification of Ogawa, Inaba, Hikojima and NAG (Non-agglutinable ) strains, which constituted 74% , 3% , 0% and 23% of the isolated strains respectively. Antibiotic susceptibility tests showed that all the strains were sensitive to ciprofloxacin, gentamicin and doxycyclin, but there was widespread resistance to Co-trimoxazol (74%), erythromycin (64%) and tetracycline (53%). Vibrio cholerae El tor isolates were 100% positive for hly A gene amplification, but hemolytic activity was seen in 95% of the cases on blood agar.
Conclusion: Compared to previous studies, there appears to be a higher degree of resistance to tetracycline, hence there may be a need to switch to other agents for the treatment of cholera. The predominant serotype and the antibiotic resistance pattern in Kashan were different from those seen in other cities.
Materials and Methods: One hundred stool samples were collected from the patients with cholera in different cities, including Tehran, Kashan, Kermanshah and Ahwaz. The specimens were subjected to a series of diagnostic tests. Strains were identified by serotyping and their antibiotic resistance profiles were defined through susceptibility tests. We also used a polymerase chain reaction (PCR) assay to identify the hly A gene.
Results: Group-specific antisera were used for identification of Ogawa, Inaba, Hikojima and NAG (Non-agglutinable ) strains, which constituted 74% , 3% , 0% and 23% of the isolated strains respectively. Antibiotic susceptibility tests showed that all the strains were sensitive to ciprofloxacin, gentamicin and doxycyclin, but there was widespread resistance to Co-trimoxazol (74%), erythromycin (64%) and tetracycline (53%). Vibrio cholerae El tor isolates were 100% positive for hly A gene amplification, but hemolytic activity was seen in 95% of the cases on blood agar.
Conclusion: Compared to previous studies, there appears to be a higher degree of resistance to tetracycline, hence there may be a need to switch to other agents for the treatment of cholera. The predominant serotype and the antibiotic resistance pattern in Kashan were different from those seen in other cities.
Keywords: Vibrio cholera el tor
Type of Study: Research |
Subject:
General
Received: 2005/05/23 | Accepted: 2006/02/22 | Published: 2013/08/11
Received: 2005/05/23 | Accepted: 2006/02/22 | Published: 2013/08/11
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