Volume 10, Issue 4 (18 2013)                   sjsph 2013, 10(4): 33-44 | Back to browse issues page

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Mohammadian Y, Shahtaheri S J, Sabour yaraghi A A, Kakooei H, Hajaghazadeh M. Determination of toxicological indexes of carbon nanotubes and Chrysotile according to invitro cytotoxicity  on Human Lung epithelium cells. sjsph. 2013; 10 (4) :33-44
URL: http://sjsph.tums.ac.ir/article-1-3-en.html
1- , shahtaheri@tums.ac.ir
Abstract:   (8310 Views)

Background and aim: In this study the cytotoxicity to human epithelial lung cells of single-walled carbon nanotubes, multi-walled carbon nanotubes and chrysotile was compared based on the following cytotoxicity indices: no observable adverse effect concentration (NOAEC), inhibitory concentration 50 (IC50), and Total Lethal Concentration (TLC).

Materials and Methods: Human epithelial lung cells were exposed to different concentrations (1 to 1500 µg/ml) of carbon nanotubes and chrysotile for 6 and 24 hours. Cytotoxicity was assessed using the MTT assay. NOAEC, IC50, and TLC idices were determined by probit analysis.

Results: The results showed statistically significant correlations (p<0.001) between cytoxicity and exposure concentration in the case of all the three compounds. The NOAEC and IC50 indices were lowest for the multi-walled carbon nanotubes, while the single-walled carbon nanotubes showed the lowest TLC index.

Conclusion: Cytotoxicity of multi-walled carbon nanotubes at low concentrations was higher than that of single-walled carbon nanotubes and chrysotile. This would mean that exposure to this compound occurs at low concentrations. Thus, cytotoxicity of multi-walled carbon nanotubes is a cause for concern. It can be concluded, then, that, like chrysotile fibers, crabon nanotubes are also considerably toxic to human epithelial lung cells.

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Type of Study: Research | Subject: General
Received: 2012/09/2 | Accepted: 2013/01/15 | Published: 2013/07/24

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