Volume 22, Issue 4 (3-2025)
sjsph 2025, 22(4): 441-450 |
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Tajmahal M, Rahimi E. Investigation of the Prevalence of Coxiella Burnetii in Traditional Cheese and Raw Milk Supplied in Qom City, Iran Using the Nested PCR Method. sjsph 2025; 22 (4) :441-450
URL: http://sjsph.tums.ac.ir/article-1-6373-en.html
URL: http://sjsph.tums.ac.ir/article-1-6373-en.html
1- MSc. Department of Food Hygiene, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran, MSc. Department of Food Hygiene, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Ph.D. Professor, Department of Food Hygiene, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran ,ebrahimrahimi55@yahoo.com
2- Ph.D. Professor, Department of Food Hygiene, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran ,
Abstract: (1337 Views)
Background and Aim: Q fever, caused by Coxiella burnetii, is a zoonotic disease widely distributed in humans and livestock. It affects domestic animals, wildlife and humans. This disease is asymptomatic in livestock and its clinical consequences include abortion, stillbirth, infertility, mastitis and endometritis. Unpasteurized milk and dairy products are the most important source of transmission of C. burnetii to humans. The aim of the present study was to investigate the prevalence of C. burnetii in traditional cheese and raw cow's, sheep's, goat's and buffalo’s milks
supplied in Qom city, Iran using the Nested PCR method.
Materials and Methods: Initially 150 samples including 30 samples of traditional cheese and 120 samples of raw cow's, sheep's, goat's and buffalo’s milks were selected from the supply centers in Qom city in the summer of 2024 and transferred to the laboratory under sterile conditions to prevent secondary contamination. The samples were tested using the Nested PCR method to detect contamination.
Results: The results showed that out of a total of 120 samples of raw ruminant milk and 30 samples of traditional cheese, 8 samples (5.33%) were contaminated with Coxiella burnetii. The proportions of contaminated samples in cow's, sheep's and goat's milks and traditional cheese were 3 (2%), 1 (0.67%), 1 (0.67%) and 3 (2%), respectively; buffalo raw milk was not contaminated.
Conclusion: Based to the results of the present study, it is recommended to prohibit the consumption of raw dairy products to reduce the risk of pathogenicity and infection by Coxiella burnetii.
supplied in Qom city, Iran using the Nested PCR method.
Materials and Methods: Initially 150 samples including 30 samples of traditional cheese and 120 samples of raw cow's, sheep's, goat's and buffalo’s milks were selected from the supply centers in Qom city in the summer of 2024 and transferred to the laboratory under sterile conditions to prevent secondary contamination. The samples were tested using the Nested PCR method to detect contamination.
Results: The results showed that out of a total of 120 samples of raw ruminant milk and 30 samples of traditional cheese, 8 samples (5.33%) were contaminated with Coxiella burnetii. The proportions of contaminated samples in cow's, sheep's and goat's milks and traditional cheese were 3 (2%), 1 (0.67%), 1 (0.67%) and 3 (2%), respectively; buffalo raw milk was not contaminated.
Conclusion: Based to the results of the present study, it is recommended to prohibit the consumption of raw dairy products to reduce the risk of pathogenicity and infection by Coxiella burnetii.
Type of Study: Research |
Subject:
Public Health
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