Volume 14, Number 3 (12-2016)                   sjsph 2016, 14(3): 85-96 | Back to browse issues page


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Nargesi S, Koordbacheh P, Farahyar S, Noorbakhsh F, Rezaie S. The Effects of Diclofenac Sodium on Growth and Morphological Changes in Aspergillus Fumigatus and Exploring its Effects on Siderophore Protein by Determining the SidB Gene Expression. sjsph. 2016; 14 (3) :85-96
URL: http://sjsph.tums.ac.ir/article-1-5411-en.html

1- MSc. Department of Medical Mycology and Parasitology, School of public Health, Tehran University of Medical Sciences, Tehran, Iran
2- Ph.D. Professor, Department of Medical Mycology and Parasitology, School of public Health, Tehran University of Medical Sciences, Tehran, Iran
3- Ph.D. Assistant Professor, Department of Medical Mycology and Parasitology, School of Medicin, Iran University of Medical Sciences, Tehran Iran
4- Ph.D. Assistant Professor, Department of Biology, Islamic Azad University, Varamin-Pishva Branch, Varamin, Iran
5- Ph.D. Professor, Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , srezaie@tums.ac.ir
Abstract:   (1499 Views)

Background and Aim: Considering the increasing drug resistance to Aspergillus fumigatus,

search for genes involved in its pathogenicity and identifying alternative antifungal drugs is of utmost importance. The aim of this study was to determine the effects of diclofenac sodium on the growth and sidB gene expression in Aspergillus fumigatus.

Materials and Methods: In this study, Aspergillus fumigatus was cultured and a fungal suspension prepared, followed by determining the minimum inhibitory concentration of diclofenac sodium at a concentration of 25-1000 µg/ml. Then, RNA was extracted from the suspension at concentrations of 500,700 and 900 µg/ml of the drug. Finally, the extent of expression of the gene was determined by measuring different levels of mRNA-sidB by Real-time PCR.

Results: With increasing concentrations of diclofenac sodium, mycelium production decreased. Concentrations higher than 500 µg/ml had considerable inhibitory effects on the growth of Aspergillus fumigatus.

Conclusion: Findings of this study indicate that diclofenac sodium can cause a sharp reduction in the growth rate of Aspergillus fumigatus. Accordingly, it can be considered as one of the effective pharmacological agents for inhibiting the growth of this fungus.

Full-Text [PDF 979 kb]   (498 Downloads)    
Type of Study: Research | Subject: Public Health
Received: 2016/12/21 | Accepted: 2016/12/21 | Published: 2016/12/21

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